SCIENTIFIC JOURNALS المجلات العلمية

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Anti-inflammatory Activity of the Plant Cannabis sativa (L) Petrolium Ether Extract in Albino Rats
(جامعة الشيخ عبدالله البدري, 2011) Elbadri E. Osman
In this study the plant Cannabis sativa seeds petroleum oil extract was investigated for anti- inflammatory activity on albino rats. The inflammation was firstly obtained by using carrageenan suspension 0.1 ml of 10% saline injected at the sub – plantar region of the left limb for inducing a local acute oedema. A decreased in oedema size was reported after 24 hours for the rats pretreated with carrageenan30 minutes before injection with suspension( 4.56, 0.59 and 0.93 for control, 1ml/kg per day and 0.5ml/kg per day groups given C. sativa seed extracts respectively.), compared to Indomethacin standard antiinflammatory drug which reported a decrease in oedema size diameter to 0.55mm, which indicated an increase inhibition percentages were reported for the different pretreated groups 0.00, 87.03, 79.56 and 87.91 including the comparative Indomethacin treated groups of rats respectively. On the other hand, the post-treated groups of rats (given C. sativa oil extract after 30 minutes of injection of suspension) showed a similar results for maximum concentration 1 ml/day of C. sativa oil extract in comparison to the standard drug. Hence, such results recommend the prospect focus for the preventive medication use of the extract. The study also highlights no significant changes for serum and protein of the blood taken from rats of the experiments. Although there were significant decrease in lymphocyte and neutrophil, but the changes were not significant. Indomethacin was given to the rats used for a comparative drug (10mg/kg). Moreover, the drug indomethacin used as a comparative parameter showed similar results in comparison to the extract, hence wise the reported results may be recommended for use as anti-inflammatory agent and should be explored more to formulate drug on basis of its activity.
Antiamoebic activity and cytotoxicity of ethanolic extract of Cyperus rotundus L.
(جامعة الشيخ عبدالله البدري, 2015) El-badri E. Osman
ABSTRACT Cyperus rotundus L. (Family: Cyperaceae), has a number of pharmacological and biological activities including anti-inflammatory, antidiabetic, antidiarrhoeal, cytoprotective, antimutagenic, antimicrobial, antioxidant, cytotoxic and apoptotic, anti-pyretic and analgesic activities. The World Health Organization (WHO) estimates that the protozoan Entamoeba histolytica is a major cause of morbidity worldwide, causing approximately 50 million cases of dysentery and 100,000 deaths annually. Intestinal amoebiasis due to the infection of E. histolytica is ranked third on the list of parasitic protozoan infections leading to death behind malaria and schistosomiasis. The purpose of the paper was to investigate the in-vitro antiamoebic activity and cytotoxicity (MTT assay) of ethanol extract of C. rotundus L. (whole plant). The ethanol extract of C. rotundus (whole plant), with different concentration (500, 250 and 125 ppm) and metronidazole concentration (312.5 μg/ml) was investigated in vitro against E. histolyica trophozoites. The result was obtained from C. rotundus whole plant ethanol extract which exhibited 100% mortality within 96 h, at a concentration 500 ppm; this was compared with Metronidazole which gave 96% inhibition at concentration 312.5 µg/ml at the same time. In addition cytotoxicity (MTT assay) with different concentration (500, 250 and 125 ppm) in comparison to triton-x100 (the reference control) which verified the safety of the examined extract with an IC50 less 100 µg/ml. In conclusion, these studies prove the potent activity of C. rotundus against E. histolyica trophozoites in vitro with verified safety evidence for use.
ANTIAMOEBIC AND CYTOTOXIC ACTIVITY OF BAUHINIA RUFESCENS (LAM) LEAF EXTRACTS
(جامعة الشيخ عبدالله البدري, 2015) Elbadri E. Osman
ABSTRACT The World Health Organization estimates that the protozoan is a major cause of morbidity worldwide, causing approximately 50 million cases of dysentery and 100,000 deaths annually. Intestinal amoebiasis due to the infection of E. histolytica is ranked third on the list of parasitic protozoan infections leading to death behind malaria and schistosomiasis. Metronidazole is used as a drug of choice against giardiasis. However, like a lot of other chemical agents, this drug has its own side effects. The present study was carried out to evaluate antiamoebic activites (Entamoeba histolyica) and cytotoxicity (MTT assay) of Bauhinia rufescens. Variety supreme court leaf petroleum ether and methanolic extracts. The highest activity against Entamoeba histolytica, with respect to time, was obtained from petroleum ether extract which exhibited 78.33% mortality within 72 h in 1000 ppm concentration, followed by the same extract which exhibited 75.12% mortality within 72 h with concentration of 500 ppm. On the other hand the lowest antiamoebic activity was recorded by methanol ether extract 60.20% mortality with 125 ppm concentration in 72 hours. The cytotoxicity of petroleum ether and methanol extracts had varying degrees of toxicity to Vero cell lines with IC50 391.39μg/ml for the petroleum ether extract and 488.32μg/ml for the methanol extract. These studies conducted for Bauhinia rufescens leafs was proved to have potent activities against Entamoeba histolyica trophozoites in vitro. And MTT assay verified the safety. Key Words: Bauhinia rufescens, Entamoeba histolyica, Cytotoxicity, Antiamoebic
Antiamoebic and Cytotoxicity of Ethanolic Fruit Extract of Kigelia africana
(جامعة الشيخ عبدالله البدري, 2015) Elbadri E. Osman
Kigelia africana (family Bignoniaceae), is a common component of the pharmacopeia’s of multiple African groupings which inhabit the areas in which it grows. Amongst these groups there is a myriad of medicinal uses in the treatment of a wide variety of bacterial, fungal and protozoal infections, as well as in the treatment of cancers. Intestinal amoebiasis due to the infection of E. histolytica is ranked third on the list of parasitic protozoan infections leading to death behind malaria and schistosomiasis. This study was carried out to evaluate antiamoebic activites (E. histolyica) and cytotoxicity (MTT assay) of ethanol extract of K. africana (fruits). The extract of K. africana (fruits), with different concentrations (500, 250 and 125 ppm) and Metronidazole concentration (312.5 μg/ml) to be investigated in vitro against E. histolyica trophozoites, and cytotoxicity (MTT) assay with test concentrations and Triton-100 (the reference control) was studied. The ethanolic fruit extract exhibited 100% mortality within 96 h, at a concentration 500 ppm, whereas, metronidazole gave 96% inhibition at concentration 312.5 µg/ml. MTT assay verified the safety of the examined extract. The studies conducted for K. africana fruit extract was proved to have potent activities against E. histolyica trophozoites in vitro
Antigiardial Activity and Toxicological Exploration of Cannabis Sativa Extracts
(جامعة الشيخ عبدالله البدري, 2013) Elbadri E. Osman
Abstract— The present study has been attempt to elucidate antigiardial activity and explore the cytoxicity, investigation on liver Diagnostic Enzymes and Changes in serum constituents of Cannabis Sativa aerial parts and seeds, which were extracted by Petroleum ether and methanol. Aerial parts methanolic extract gave 63.6% mortality after 72 hours at concentration 1000 ppm (IC50 0.13 ppm) comparing with metrondizole (IC50 0.0125 ppm). While the other extracts found inactive as antigiardiasis after 72 hours. The slight increase in Aspartate amino transferase (AST), Alanine amino transferase (ALT) and Alkaline phosphatase (ALP) liver enzyme and total protein, urea, albumin and calcium which indicate some degree hepatic nefropathy effect of such plant petroleum ether extract. Moreover, phytochemical examination was carried out firstly and revealed that, the petroleum ether extract of Cannabis sativa seed do not contain tetrahydrocannbinol (THC), cannabinol (CBN) and cannabidaiol (CBD). However, all extracts showed no significant cytotoxic activity against vero cell line. Index Terms— Giardia lamblia, Cytotoxicity, Cannabis sativa, Liver enzymes
Antigiardial, Amoebicidal and Cytotoxic activity of the plant Prosopis juliflora leave extracts
(جامعة الشيخ عبدالله البدري, 2014) Elbadri E. Osman
he present study was carried out to evaluate antigiardial, amoebicidal activity and explore the cytotoxicity of Prosopis juliflora (Leaves) Variety supreme court Leaves petroleum ether and methanolic extracts in vitro. Tested were performed using four concentrations: (1000 ppm, 500 ppm, 250 ppm and 125 ppm). The highest activity against Giardia lamblia, with respect to time, was obtained from petroleum ether extract which exhibited 78.91% mortality within 72 h with a concentration of 500 ppm followed by the methanolic extract which exhibited 77.48% mortality within 72 h with a concentration of 1000 ppm. On the other hand the lowest antigiardial activity was recorded by petroleum ether extract 38.55 % mortality with 1000 ppm concentration in 24 hours. The highest activity against Entamoba histolytica, with respect to time, was obtained from methanolic extract which exhibited 71.97% mortality within 72 h with a concentration of 1000 ppm. On the other hand the lowest antiamobic activity was recorded by petroleum ether extract 31.88% mortality with 125 ppm concentration within 24 hours. The cytotoxicity of methanol and petroleum ether extract had varying degrees of toxicity to vero cell lines with IC50 731.79 µg/ml for the methanol extract and 11.22 µg/ml for the petroleum ether extract.
Antigiardial, antiamoebic and cytotoxic activity of the leaves extracts of Vitex trifolia
(جامعة الشيخ عبدالله البدري, 2015) Elbadri E. Osman
ABSTRACT The World Health Organization ranks diarrheal disease as the second (after acute respiratory infections) most common cause of morbidity and mortality in children in the developing world. Entamoeba histolytica and Giardia lamblia are two of the most important and most widespread diarrhea-related parasitic protozoa in the world. Metronidazole is used as a drug of choice against amoebiasis and giardiasis. However, like a lot of other chemical agents, this drug has its own side effects. The present study was carried out to evaluate antigiardial, antiamoebic activity and explore the cytotoxicity of Vitex trifolia (leaves) variety supreme court leaves petroleum ether and methanolic extracts in vitro. The highest activity against Giardia lamblia, with respect to time, was obtained from petroleum ether extract which exhibited 75.25% mortality within 72 h with a concentration of 1000 ppm followed by the same extract which exhibited 72.07% mortality within 72 h with a concentration of 500 ppm. On the other hand, the lowest antigiardial activity was recorded by petroleum ether extract 58.92% mortality with 125 ppm concentration in 72 h. The highest activity against Entamoba histolytica, with respect to time, was obtained from methanolic extract which exhibited 61.64% mortality within 72 h with a concentration of 1000 ppm. On the other hand, the lowest antiamobic activity was recorded by petroleum ether extract 53.62% mortality with 125 ppm concentration within 72 h. The cytotoxicity of methanol and petroleum ether extract had varying degrees of toxicity to Vero cell lines with IC50 349.07 μg/ml for the methanol extract and 369.77 μg/ml for the petroleum ether extract. Keywords: Vitex doniana (Leaves), antigiardial, antiamoebic, cytotoxicity, Sudan
Antigiardial, antioxidant activities and cytotoxicity of ethanolic extract of leaves of Acacia nilotica (L)
(جامعة الشيخ عبدالله البدري, 2015-03) Ahmed Saeed Kabbashi; Mohammed Ismail Garbi; Elbadri E. Osman
Acacia nilotica (L) related to (family Fabaceae-Mimosoideae). The division is Magnolophyta and class is Magnolipsida. The genus is Acacia and species is nilotica. The ailments treated by this plant include colds, congestion, fever, gallbladder, hemorrhage, hemorrhoids, leucorrhoea, ophthalmic, sclerosis and small pox. Acacia bark is drunk for intestinal pains and used for treating acute diarrhea. Other preparations are used for gargle, toothache, ophthalmic and syphilitic ulcers. The roots of Acacia are used to treat tuberculosis. This study was carried out to evaluate antigiardial activity (Giardia lamblia), antioxidant (DPPH assay) and cytotoxicity (MTT assay) of ethanol extract of A. nilotica (leaves). The ethanol extracts of A. nilotica (leaves) was screened for its antigiardial activities (Giardia lamblia), antioxidant screening for their free radical scavenging properties using 2.2Di (4-tert-octylphenyl)-1-picryl-hydrazyl (DPPH), while propyl gallate was used as standard antioxidant and screened for their cytotoxicity using 3-(4, 5-dimethyl thiazol-2-yl)-2, 5- diphenyl tetrazolium bromide (MTT), was obtained from A. nilotica (leaves) ethanol extract which exhibited 100% mortality within 96 h, at a concentration 500 µg/ml; this was compared with Metronidazole which gave 96% inhibition at concentration 312.5 µg/ml at the same time. The tested antioxidant activity gave (65 ± 0.04 RSA%) in comparison to the control of propyl gallate levels (88 ± 0.07RSA%), and MTT assay verified the safety of the examined extract. In conclusion: These studies conducted for both A. nilotica (leaves) was proved to have potent activities against Giardia lamblia trophozoites in vitro.
Antigiaridial Activity and Cytotoxicity of Ethanolic Bark Extract of Acacia nilotica (L.)
(جامعة الشيخ عبدالله البدري, 2016) El-badri E. Osman
Abstract: Background: Acacia nilotica (L.) was used to treat different ailments for instance cold, congestion, fever, gallbladder, hemorrhage, hemorrhoids, leucorrhoea, ophthalmic, sclerosis, small pox, intestinal pains and acute diarrhea. Other preparations are used for gargle, toothache, ophthalmic and syphilitic ulcers. Human parasitic infections still represent a challenging public health problem worldwide, especially in tropical and subtropical regions. Giardiasis is the most common cause of parasitic gastro-intestinal disease and up to two hundred million people are chronically infected with Giardia lamblia globally with 500,000 new cases reported annually. Objectives: The purpose of the paper is to investigate the in-vitro antigiardial activity and cytotoxicity (MTT assay) of ethanol extract of A. nilotica subsp. nilotica (bark). Method: The ethanolic extract of A. nilotica (bark), with different concentrations (500, 250 and 125 ppm) were investigated together with Metronidazole as a reference control at 312.5 μg/ml against Giardia lamblia trophozoites. Cytotoxicity (MTT assay) was also performed with different concentrations (500, 250 and 125 ppm) and compared to the reference control Triton-x100. Result: A. nilotica bark ethanolic extract exhibited 100% mortality within 96 h, at a concentration of 500 ppm; this was compared with Metronidazole which gave 96% inhibition at the concentration of 312.5 μg/ml at the same time. In addition cytotoxicity (MTT-assay) verified the safety of the examined extract with an IC50 less than 100 μg/ml. Conclusion: These studies prove the potent activity of A. nilotica against Giardia lamblia trophozoites in vitro with verified safety evidence for use. .
ANTIOXIDANT ACTIVITY AND PHYTOCHEMICAL SCREENING OF THE METHANOLIC LEAVES EXTRACT OF B RUFESCENS (LAM)
(جامعة الشيخ عبدالله البدري, 2015) Elbadri E. Osman
ABSTRACT Interest in natural products as a source for innovation in drug discovery and agrochemicals is still growing worldwide. Natural products, whose immense diversity has been appreciated for many years, may become in a rich source of novel chemical structures. The present study was conducted to investigate the in-vitro antioxidant (DPPH assay) and phytochemical screening of methanol extract of B rufescens (leaves). The methanol extract of B. rufescens (leaves) was tested for antioxidant screening for their free radical scavenging properties using 2.2Di (4-tert-octylphenyl)-1-picryl-hydrazyl (DPPH), while propyl galate was used as standard antioxidant and phytochemical screening. The methanol extract of B rufescens (leaves) antioxidant activity was (81 ± 0.04 RSA%) in comparison to the control of propyl galate levels (88 ± 0.07RSA%) and Preliminary phytochemical screening of the leaves of B rufescens revealed that the plant contain triterpenes, alkaloids, tannins, saponins and flavonoids. Negative results were recorded for glycosides and coumarins. Hence, the results obtained in the present study indicate that B rufescens have promising antioxidant indicates that the plant could be promising agent in scavenging free radicals and treating diseases related to free radical reactions. Keywords: Antioxidant (DPPH-assay), Phytochemical, B rufescens (leaves)
Antioxidant activity and phytochemical screening of methanolic leaves extract of Bauhinia rufescens (Lam)
(جامعة الشيخ عبدالله البدري, 2015) Elbadri E. Osman
Abstract Interest in natural products as a source for innovation in drug discovery and agrochemicals is still growing worldwide. Natural products, whose immense diversity has been appreciated for many years, may become in a rich source of novel chemical structures. Our country is a rich source of both biological and chemical diversity, which may be useful as a source of novel chemical structures. The present study was conducted to investigate the in-vitro antioxidant (DPPH assay) and phytochemical screening of methanol extract of Bauhinia rufescens (leaves). The methanol extract of B. rufescens (leaves) was tested for antioxidant screening for their free radical scavenging properties using 2.2Di (4- tert-octylphenyl)-1-picryl-hydrazyl (DPPH), while propyl galate was used as standard antioxidant and phytochemical screening. The methanol extract of B. rufescens (leaves) antioxidant activity was (81 ± 0.04 RSA%) in comparison to the control of propyl galate levels (88 ± 0.07RSA%) and Preliminary phytochemical screening of the leaves of B. rufescens revealed that the plant contain Triterpenes, alkaloids, Tannins, Saponins and Flavonoids. Negative results were recorded for glycosides and Coumarins. Hence, the results obtained in the present study indicate that B. rufescens have promising antioxidant agents; it indicates that the plant could be a promising agent in scavenging free radicals and treating diseases related to free radical reactions. Keywords: Antioxidant, (DPPH), Phytochemical, Bauhinia rufescens
Antioxidant activity, phytochemical screening and cytotoxicity of ethanolic leaves extract of Antigonon leptopus
(جامعة الشيخ عبدالله البدري, 2015) Elbadri E. Osman
he leaves of Antigonon leptopus, belonging to the family Polygonaceae. A. leptopus is used as a treatment for cough and throat constriction in Sudan and considered as one of the important medicinal plants in their folk-medicine. The present study investigated the in-vitro antioxidant (DPPH assay), phytochemical screening and cytotoxicity (MTT) of ethanol extract of A. leptopus (leaves). The ethanol extract of A. leptopus (leaves) was screened for detect their free radical scavenging properties using 2.2Di (4-tert-octylphenyl)-1-picryl-hydrazyl (DPPH), while propyl galate was used as a standard antioxidant and screened for their cytotoxicity using 3- (4, 5-dimethyl thiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT). Ethanol extracts of A. leptopus (leaves). The tested antioxidant activity gave (89 ± 0.04 RSA%) in comparison to the control of propylgalate levels (72 ± 0.01RSA%), Primary phytochemical screening on leaves of A. leptopus appeared the rich of alkaloids, saponins, tannins, unsaturated sterol and/or triterpenes, flavonoids, glycosides and coumarins. And MTT assay appeared the safety of the extract. Key words: Antioxidant activities (DPPH), phytochemical screening and cytotoxicity (MTT), Antigonon leptopus (leaves)
Antioxidant and antiglycation potential of some Sudanese medicinal plants and their isolated compounds
(جامعة الشيخ عبدالله البدري, 2009) Elbadri E. OSMAN
Free radicals or reactive oxygen species (ROS) appear to be associated with a number of human neurodegenerative disorders, inflammation, diabetes, viral infections, autoimmune pathologies and digestive system disorders. In the present work twenty three ethanolic extracts of 20 medicinal plants, commonly used in Sudanese folk medicines against infectious diseases were investigated for their potential scavenging of superoxide free radicals. 14 extracts showed significant activity P < 0.05 for the scavenging of superoxide free radicals. Acacia nilotica bark, Balanites aegyptiaca barks, Khaya senegalensis leaves were the most active with 75, 72, 71% inhibition, respectively. However, the rest revealed moderate inhibition activity. Only A. nilotica barks (78%), K. senegalensis barks (74%), A. nilotica fruits (66%) and Tinspora bakis (61%) showed over 50% inhibition of glycation production assay, while the rest were less effective. Bioassay-guided phytochemical investigation proved catechin was the most active isolated secondary metabolite for both scavenging of superoxide free radicals and inhibition of glycation production assay. MTT cytotoxicity against 3T3 cell line indicates the safety of all plant ethanolic extracts as well as isolated compounds.
Antioxidant and antiglycation potential of some Sudanese medicinal plants and their isolated compounds
(جامعة الشيخ عبدالله البدري, 2009) Elbadri E. OSMAN
Abstract Free radicals or reactive oxygen species (ROS) appear to be associated with a number of human neurodegenerative disorders, inflammation, diabetes, viral infections, autoimmune pathologies and digestive system disorders. In the present work twenty three ethanolic extracts of 20 medicinal plants, commonly used in Sudanese folk medicines against infectious diseases were investigated for their potential scavenging of superoxide free radicals. 14 extracts showed significant activity P < 0.05 for the scavenging of superoxide free radicals. Acacia nilotica bark, Balanites aegyptiaca barks, Khaya senegalensis leaves were the most active with 75, 72, 71% inhibition, respectively. However, the rest revealed moderate inhibition activity. Only A. nilotica barks (78%), K. senegalensis barks (74%), A. nilotica fruits (66%) and Tinspora bakis (61%) showed over 50% inhibition of glycation production assay, while the rest were less effective. Bioassay-guided phytochemical investigation proved catechin was the most active isolated secondary metabolite for both scavenging of superoxide free radicals and inhibition of glycation production assay. MTT cytotoxicity against 3T3 cell line indicates the safety of all plant ethanolic extracts as well as isolated compounds.
Constituents and Antimicrobial Activity of Sudanese Azadirachta indica (Meliaceae) Fixed Oil
(جامعة الشيخ عبدالله البدري, 2019) Abdel Karim, M.1*,; AbdelAzim, I.1; andUm-Alahasan,T.2
Abstract: In this study Azadirachta indica fruit oil was studied by GC-MS. The oil was also assessed for antimicrobial activity. The GC-MS analysis revealed the presence of 29 constituents .The major constituents of the oil are: i) 9-octadecenoic acid methyl ester (37.20%) , ii) methyl stearate(20.42%) , iii) hexadecanoic acid methyl ester(19.13%) and iv) 9, 12-octadecadienoic acid methyl ester (12.60%).The antimicrobial activity of the oil was evaluated via the agar diffusion bioassay against five standard pathogenic bacteria (Gram positive: Staphylococcus aureus and Bacillus subtilis; Gram negative: Esherichia coli, Pseudomonasaaeruginosa) and the fungus Candida albicans. At a concentration of 100mg/ml the oil showed good activity against Escherichia coli. However, at the same concentration, it exhibited partial activity against Staphylococcus aureus and Bacillus subtilis. The oil failed to give any anticandidal activity
Cytotoxicity of Vitex trifolia leaf extracts on MCF-7 and Vero cell lines
(جامعة الشيخ عبدالله البدري, 2015) Elbadri E. Osman
Pharmacological and preventive properties of Vitex trifolia leaf extracts are well known, but the anticancer activity of methanol and petroleum ether extracts of Vitex trifolia leafs on human breast cancer cells have not been explored so far. Therefore, the present study was designed to investigate the cytotoxic activities of these extracts against MCF-7 and Vero cell line. Cells were exposed to 125 to 500 μg/ml of the extracts of Vitex trifolia for 72 h. Post-treatment, percent cell viability was studied by 3-(4, 5-dimethylthiazol-2yl)-2, 5-biphenyl tetrazolium bromide (MTT) assays. The results showed that petroleum ether and methanol extracts significantly reduced cell viability of MCF-7 cells in a concentration dependent manner. Concentrations of 125 μg/ml and above of petroleum ether and 500 μg/ml of methanol extract were found to be cytotoxic in MCF-7 cells. Cell mortality at 125, 250 and 500 μg/ml of petroleum ether extract was recorded as 98.80%, 93.40% and 87.75% respectively, whereas at 125, 250 and 500 of methanol extract values were 79.98%, 75.70% and 70.25%, respectively by MTT assay. MCF-7 and Vero cells exposed to 125, 250 and 500 μg/ml. The data revealed that the treatment with petroleum ether and methanol of Vitex trifolia leaf extract induced cell death in MCF-7 cells. Meanwhile the same extract showed a moderate inhibition against Vero cell lines. It may be concluded that Vitex trifolia can cause cell death in MCF-7 cancer cells, which can be considered as a promising chemotherapeutic agent in breast cancer treatment. Keywords: Vitex trifolia, MCF-7, Vero, Cytotoxicity, MTT
Detection of Asymptomatic Bacteriuria and Antimicrobial Susceptibility Testing among Diabetic Patients in Khartoum State
(جامعة الشيخ عبدالله البدري, 2023) Hadia Babiker Abdelbaset1,; Abdulla1Ghanem Mohammed Mahjaf2; Amna M. Ahmed1; Abrar B. Omer1; Athar T. Mohammed1; Sara B; , Mosab Nouraldein Mohammed Hamad3*
Abstract: Background: Urinary tract infections (UTIs) are one of the most common infections seen in all age groups with diabetes mellitus (DM). The term asymptomatic bacteriuria (ASB) refers to the isolation of bacteria in a urine specimen of individuals with unobserved symptoms of UTIs. DM is one of the risk factors of UTIs and causes complications including renal abscess, cystitis, fungal infections, pyelonephritis, and renal papillary necrosis. Objectives: This study aimed to detect asymptomatic bacteriuria and antimicrobial susceptibility patterns for isolated organisms among adult, asymptomatic diabetic patients were attended selected diabetic hospitals and centers in Khartoum state. Methods: A descriptive, prospective cross-sectional study was conducted on 120 asymptomatic diabetic patients from July to October 2022 in Khartoum state, Sudan. Information about patient demographics and clinical status was obtained from each patient using a written questionnaire. Clean-catch midstream urine specimens were collected and cultured on CLED, then processed for isolation and identification of uropathogens through conventional microbiological procedures. Antibiotic susceptibility patterns were determined by using the Kirby-Bauer disc diffusion method through culturing the isolates on Mueller- Hinton agar. The collected data and laboratory results were analyzed using SPSS version 26. Results: 120 asymptomatic diabetic patients were included in this study, the age -mean was 44.8±11.76, out of which 16.7% (n=20/120) showed significant ASB and 75%(n=15/20) of them were females. In this study, there was a significant association between the level of HbA1c (P. value 0.049), bacteriuria (P. value 0.000), and ASB among studied diabetic patients, on the other hand, no significant association between age, gender, or type of DM, duration of DM, recurrent UTIs, other study variables and ASB. S. aureus was the commonest isolated uropathogen (40%) followed by P. aeruginosa (25%), E. coli (15%), E. faecalis (15%), C. koseri (5%), S. aureus isolates were resistant to oxacillin in (62%). The isolated organisms were resistant to cefotaxime (50%), gentamycin (50%), imipenem (35%), nalidixic acid (75%) ciprofloxacin (40%). Conclusion: The overall prevalence of ASB among asymptomatic diabetic patients was high (16.7%). In this study poor glycemic control is a significant risk factor for ASB. Conclusion: Regular screening for ASB through culture and antimicrobial susceptibility testing is recommended mainly for females over 45 years.
Detection of secretor gene among Sudanese patients with chronic renal failure in Shendi town: A cross sectional study
(جامعة الشيخ عبدالله البدري, 2023) Nazzla Abd Alhameed H. Mohammed1; Elfatih Mohammed A. Ali1,; Alaa Babiker H. Alkab1; Ghanem Mohammed Mahjaf2; Tibyan Abdalmajed Altaher3; Mosab Nouraldein Mohammed Hamad4*
Abstract: Background: Secretor status is valuable with some diseases in clinical and forensic medicine. Objectives: This study aimed to evaluate the status of secretors and non-secretors in chronic renal failure patients under hemodialysis in Shendi town at the kidney treatment and surgery center (Sudan). Method: Saliva samples were collected from fifty patients (58% were male) aged from 15 to 65 years and secretor status was studied by the hemagglutination inhibition method of saliva. Result: results showed that 68% of the study population were non-secretors. probable frequency of the Se and se genes was 17,6% and 82,4% while the frequency of SeSe, Sese, and sese allele genes was 3%, 29%, and 68% respectively. were calculated by utilizing Hardy-Weinberg Theorem for probabilities and possibilities. Frequencies of the non-secretor status among various ABO blood groups were 63.2% in group A, 75% in group B, and 72.7% in group O. Conclusion: We conclude that non-secretors are more prevalent in chronic renal failure. In the present study observed that non-secretors were more common as compared to secretors, non-secretors are more prone to renal failure.
Diversity of Ants in North Kordofan, Sudan
(جامعة الشيخ عبدالله البدري, 2023-07-04) Muna Abdelaziz Mohamed Ibrahim,; Bothyna MA Ahmed,; Nazik A Mohamed; Awatif K Omer
Abstract The purpose of this study is to record the ant (Hymenoptera, Formicidae) species and their habitat preference in Algafeel area, North Kordofan. Specimen were collected by hand and/or a strip paper. Nine genera were identified to the species level. These constitutes the following 14 species: Camponotus oasium, Camponotus sericeus, Camponotus maculatus, Cataglyphis abyssinicus, Cataglyphis auratus, Messor galla, Monomorium areniphilum, Monomorium bicolor, Tetramorium sericeiventre, Pheidole escherichii, Pheidole rotundata, Trichomyrmex oscaris, Brachyponera chinensis and Megaponera analis. The following were identified to the generic level: Dorylus sp., Paratrechina sp., Lepisiota sp., Monomorium sp., and Pheidole sp. around 35% of the studied ant species were found in human dwellings. Camponotus maculatus, Paratrechina sp., Lepisiota sp., male of Messor sp., Pheidole sp. (major +minor) and Cataglyphis sp., are record for the first time in North Kordofan.
Effect of direct exposure to ringing mobile phone waves on ECG heart rate and rhythm among healthy adults
(جامعة الشيخ عبدالله البدري, 2019) Eman Z.H. yassin1, ,; Ishraga. A Ibrahim2; Omer A Elbedri3,; Samia H Abdalwahab4,,; Maria A Aboelhassan5; Alwathig Y Mohamed6
Abstract The effect of electromagnetic wave from mobile phones on human health is of great interest, the current study was designed to investigate the effect of mobile phone electromagnetic waves on ECG heart rate and rhythm among different healthy adult volunteers’ ages and sex while mobile phone was placed on the chest pocket level during ringing mode. It was an experimental, comparative study conducted in the period from march 2014 to August 2016 in north of Sudan at Elsheikh Abdallah Elbadri University among 850 volunteers, Sudanese and other nationalities.500 male and 350 females, aged 16 - 49 years were included in the study. ECG was done before and after exposure to ringing mobile phone. Significant changes in ECG heart rate and rhythm was detected and the results was found to be consistent in males and females’ group. The study conclude that mobile phone is not a safety device and should not be used by medical staff inside the ECG rooms.

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