Cytotoxicity of Vitex trifolia leaf extracts on MCF-7 and Vero cell lines
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Date
2015
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جامعة الشيخ عبدالله البدري
Abstract
Pharmacological and preventive properties of Vitex trifolia leaf extracts are well known, but the anticancer
activity of methanol and petroleum ether extracts of Vitex trifolia leafs on human breast cancer cells have not
been explored so far. Therefore, the present study was designed to investigate the cytotoxic activities of these
extracts against MCF-7 and Vero cell line. Cells were exposed to 125 to 500 μg/ml of the extracts of Vitex
trifolia for 72 h. Post-treatment, percent cell viability was studied by 3-(4, 5-dimethylthiazol-2yl)-2, 5-biphenyl
tetrazolium bromide (MTT) assays. The results showed that petroleum ether and methanol extracts
significantly reduced cell viability of MCF-7 cells in a concentration dependent manner. Concentrations of 125
μg/ml and above of petroleum ether and 500 μg/ml of methanol extract were found to be cytotoxic in MCF-7
cells. Cell mortality at 125, 250 and 500 μg/ml of petroleum ether extract was recorded as 98.80%, 93.40% and
87.75% respectively, whereas at 125, 250 and 500 of methanol extract values were 79.98%, 75.70% and
70.25%, respectively by MTT assay. MCF-7 and Vero cells exposed to 125, 250 and 500 μg/ml. The data
revealed that the treatment with petroleum ether and methanol of Vitex trifolia leaf extract induced cell death in
MCF-7 cells. Meanwhile the same extract showed a moderate inhibition against Vero cell lines. It may be
concluded that Vitex trifolia can cause cell death in MCF-7 cancer cells, which can be considered as a
promising chemotherapeutic agent in breast cancer treatment.
Keywords: Vitex trifolia, MCF-7, Vero, Cytotoxicity, MTT