Antioxidant activity, phytochemical screening and cytotoxicity of ethanolic leaves extract of Antigonon leptopus
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Date
2015
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جامعة الشيخ عبدالله البدري
Abstract
he leaves of Antigonon leptopus, belonging to the family Polygonaceae. A. leptopus is used as a
treatment for cough and throat constriction in Sudan and considered as one of the important medicinal
plants in their folk-medicine. The present study investigated the in-vitro antioxidant (DPPH assay),
phytochemical screening and cytotoxicity (MTT) of ethanol extract of A. leptopus (leaves). The ethanol
extract of A. leptopus (leaves) was screened for detect their free radical scavenging properties using
2.2Di (4-tert-octylphenyl)-1-picryl-hydrazyl (DPPH), while propyl galate was used as a standard
antioxidant and screened for their cytotoxicity using 3- (4, 5-dimethyl thiazol-2-yl)-2, 5-diphenyl
tetrazolium bromide (MTT). Ethanol extracts of A. leptopus (leaves). The tested antioxidant activity
gave (89 ± 0.04 RSA%) in comparison to the control of propylgalate levels (72 ± 0.01RSA%), Primary
phytochemical screening on leaves of A. leptopus appeared the rich of alkaloids, saponins, tannins,
unsaturated sterol and/or triterpenes, flavonoids, glycosides and coumarins. And MTT assay appeared
the safety of the extract.
Key words: Antioxidant activities (DPPH), phytochemical screening and cytotoxicity (MTT), Antigonon leptopus
(leaves)