Antigiardial Activity and Toxicological Exploration of Cannabis Sativa Extracts
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Date
2013
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جامعة الشيخ عبدالله البدري
Abstract
Abstract— The present study has been attempt to elucidate
antigiardial activity and explore the cytoxicity, investigation on
liver Diagnostic Enzymes and Changes in serum constituents of
Cannabis Sativa aerial parts and seeds, which were extracted by
Petroleum ether and methanol. Aerial parts methanolic extract
gave 63.6% mortality after 72 hours at concentration 1000 ppm
(IC50 0.13 ppm) comparing with metrondizole (IC50 0.0125 ppm).
While the other extracts found inactive as antigiardiasis after 72
hours. The slight increase in Aspartate amino transferase (AST),
Alanine amino transferase (ALT) and Alkaline phosphatase (ALP)
liver enzyme and total protein, urea, albumin and calcium which
indicate some degree hepatic nefropathy effect of such plant
petroleum ether extract. Moreover, phytochemical examination
was carried out firstly and revealed that, the petroleum ether
extract of Cannabis sativa seed do not contain tetrahydrocannbinol
(THC), cannabinol (CBN) and cannabidaiol (CBD). However, all
extracts showed no significant cytotoxic activity against vero cell
line.
Index Terms— Giardia lamblia, Cytotoxicity, Cannabis sativa,
Liver enzymes