Antiamoebic and Cytotoxicity of Ethanolic Fruit Extract of Kigelia africana
No Thumbnail Available
Date
2015
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
جامعة الشيخ عبدالله البدري
Abstract
Kigelia africana (family Bignoniaceae), is a common component of the
pharmacopeia’s of multiple African groupings which inhabit the areas in
which it grows. Amongst these groups there is a myriad of medicinal uses in
the treatment of a wide variety of bacterial, fungal and protozoal infections, as
well as in the treatment of cancers. Intestinal amoebiasis due to the infection of
E. histolytica is ranked third on the list of parasitic protozoan infections
leading to death behind malaria and schistosomiasis. This study was carried
out to evaluate antiamoebic activites (E. histolyica) and cytotoxicity (MTT
assay) of ethanol extract of K. africana (fruits). The extract of K. africana
(fruits), with different concentrations (500, 250 and 125 ppm) and
Metronidazole concentration (312.5 μg/ml) to be investigated in vitro against
E. histolyica trophozoites, and cytotoxicity (MTT) assay with test
concentrations and Triton-100 (the reference control) was studied. The
ethanolic fruit extract exhibited 100% mortality within 96 h, at a concentration
500 ppm, whereas, metronidazole gave 96% inhibition at concentration 312.5
µg/ml. MTT assay verified the safety of the examined extract. The studies
conducted for K. africana fruit extract was proved to have potent activities
against E. histolyica trophozoites in vitro