Browsing by Author "Ghanem M. Mahjaf"

Now showing 1 - 4 of 4
  • No Thumbnail Available
    Item
    Clinical Course of Asymptomatic and Mildly Symptomatic COVID-19 Patients Who had A Positive PCR Test in Multicenter in Khartoum
    (جامعة الشيخ عبدالله البدري, 2022-05-14) Marawan Izzeldin A.Abdallah; Tarig Elhadi Elsidig; Farh Ahmed Elhassan Mohamed; Ghanem M. Mahjaf; Mosab Nouraldein Mohammed Hamad
  • No Thumbnail Available
    Item
    Detection of Antifungal Activity of Cinnamon Extract on Candida Species Isolated from Diabetic Patients with Urinary Tract Infection Pre-COVID-19 Era
    (جامعة الشيخ عبدالله البدري, 2022-02-27) Waleed O.A. KaramAlla; Hadia Abass Eltaib; Ghanem M. Mahjaf; Mosab Nouraldein Mohammed Hamad
    Abstract: This study aimed to evaluate the antifungal activity of Cinnamon extract on Candida species isolated from 100 diabetic patients suffering from urinary tract infection between May to August 2018. Twenty-five Candida species were isolated, different Candida species were isolated then identified biochemically, in vitro sensitivity testing using well diffusion technique against Cinnamon extract was done. The cinnamon extract showed good antifungal activity against Candida species. The largest zone of inhibition against Candida tropicalis was (25 mm) and the least zone of inhibition was against Candida kefyr was (10 mm). The results of antimicrobial activity of crude extract were compared with the positive control (Clotrimazole) for evaluating their relative percentage inhibition and statistical significance the aqueous extract exhibited maximum relative percentage inhibition against Candida kefyr (89%) and minimum relative percentage inhibition against Candida glabrata was (12.3%). The p. values (0.018) are statistically significant.
  • No Thumbnail Available
    Item
    Detection of CTX-M Gene β-Lactamase in Gram Negative Bacteria Isolate from Shendi Health Facilities
    (جامعة الشيخ عبدالله البدري, 2022-05-24) Entisar Ayoub Ahmed1 , 1 ,1 , 2* ,; Leila M. Abdelgadeir; Ghanem M. Mahjaf; Mosab Nouraldein Mohammed Hamad; Tarig Mohamed Ahmed Mohamed
    Abstract: Background: The emergence of antibiotic resistance pathogen is an important health risk, usually gram negative bacteria acquire resistance to antibiotics by the production of beta-lactamase enzyme, CTX-M-type enzymes are a group of class A extended-spectrum B-lactamases (ESBLs) that are rapidly Spreading among gram-negative worldwide. CTX-M-type ESBLs exhibit powerful activity against cefotaxime and ceftriaxone antibiotics but generally not against ceftazidime, in some geographical areas, CTX-M is now the most prevalent ESBLs in Enterobacteriaceae. There are 172 known variants of CTX-M genes identified until August 25, 2016. Objectives: This present study aims to identify the gene, namely: CTX-M, Responsible for extended-spectrum beta-lactamase (ESBL) among Enterobacteriaceae isolated from different samples of Sudanese patients in Shendi city (urine, pus, and wound). Methodology: 200 samples were identified as gram-negative bacteria. Identification of the isolates was done by using conventional biochemical methods, and ESBLs were screened according to (CLSI) guidelines. ESBLs Positive strains were tested for the presence of ESBL encoding CTX-M, Gene by using PCR with specific primers for the detection of CTX-M. Results: ESBL found to be higher in P. aeruginosa n=18(78.3%) Klebsiella spp n=45 (64.2%). Escherichia coli n=51(57.3%) Proteus n=8(53.3%) the total of ESBL from all gram-negative isolate represent n=122 (61%). The presence of the CTX-M, the gene was confirmed in n=57(46.8%) in all isolates. The CTX-M gene were detected in n= 17 (37.8%) Klebsiella spp, n=28(55%) of Escherichia coli, n=3(37.5%) in Proteus and n=9 (50%) in P. aeruginosa isolates. Conclusion: P. aeruginosa isolates represent a high percentage of ESBL producers followed by Klebsiella spp, then Escherichia coli and proteus spp CTX-M gen detected commonly in Escherichia coli followed by P. aeruginosa then Klebsiella spp and Proteus spp. The study concludes that the ESBL production within gram-negative bacteria was high and the CTX-M gene was spread in Shendi city.
  • No Thumbnail Available
    Item
    Efficacy of Pethidine in the Treatment of Postoperative Shivering on Elective Caesarean Section Patients at Wad Madani Maternity Teaching Hospital, Gezira State, Sudan
    (جامعة الشيخ عبدالله البدري, 2022-05-24) Jamil A. Alshdadi; Mohammed K. Ahmed; Sonia A. Ahmed; Ghanem M. Mahjaf; Mosab Nouraldein Mohammed Hamad; Tarig Mohamed Ahmed Mohamed
    Abstract: Shivering is one of the most common complications of neuraxial blockade. Some patients find shivering sensation worse than surgical pain post-operatively. Therefore, both prevention and treatment of established shivering should be regarded as clinically relevant interventions during the preoperative period. This prospective experimental study was conducted at Wad Madani Maternity Teaching Hospital and aimed to measure the efficacy of pethidine when used for the treatment of postoperative shivering following spinal anesthesia during the period from November 2018 to February 2019. The sample size included of 40Patients within the American Society of Anesthesia type two physical statuses;25 mg pethidine diluted in 5 ml Normal saline-injected intra veins. All patients were monitored for temperature, heart rate, blood pressure, and oxygen saturation, these parameters were recorded preoperative, and post 10 minutes of operative, then after 10 minutes of pethidine dose administration. Data were analyzed by using a statistical package for social sciences (SPSS). The statistical results showed that there was a significant relationship between hypothermia and the presence of shivering. 90% of cases resolved to shivering, whereas 10% did not resolve, just 2.5% had Nausea and Vomiting and no side effects on the Respiratory and cardiac Systems. Based on those results the study concluded that: a small dose of pethidine 25 mg intra veins is very effective in resolving postoperative shivering with minimum side effects in the gastrointestinal tract and Good Cardiopulmonary stability. The study recommended that: warming tools should be available for patients to prevent heat loss, close monitoring of Patient's temperature intraoperative and in the recovery room, and use of 25mg Pethidine in the Presence of shivering intraoperative or post-spinal anesthesia.