The Potential Effect of Henna (Stone Dye) Extract Paraphenylenediamine on Human Blood Cells, Liver and Renal Function
No Thumbnail Available
Date
2021-12-21
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
جامعة الشيخ عبدالله البدري
Abstract
Some of the dyes that are used for coloring hair and skin contain a chemical compound called
paraphenylenediamine (PPD), which is potentially toxic. The study comprised 50 adult Sudanese
women who were selected by stratified random sampling and working as henna artist. Urine
samples were taken for detection of PPD while blood samples were taken for investigating kidney
and liver functions by measuring its relevant hematological indices. In other hand Thin Layer
Chromatography (TLC) and High Performance Liquid Chromatography (HPLC) were used to
detection of PPD in black henna (stone dye) samples and urine. The PPD was detected in the urine
of all women who used the black henna its concentration varied according to duration of exposure
being significantly higher in those with >10 years of exposure (p≤0.01). Similarly significant
changes in liver function, kidney function and hematological indices were found in the group of
women who used natural henna. This study concluded that natural henna is safe to use, while
Black dye is potentially hazardous. It contains the high concentration of PPD, is the one most
commonly associated with adverse effects.