Diagnostic tests

Abstract

Antigen preparation: - Fresh urine sample was collected from positive urinary schistosomiasis patient. - Red blood cells around S. haematobium eggs were removed by added saponin reagent. - Urine was centrifuged the sediment was washed three time by normal saline to remove saponin reagent. - Normal saline was added to the sediment and incubated over night to remove excess saponin. - After the incubation urine was centrifuged and the sediment was used as antigen for circumoval precipitin test.