Comparison and Evaluation of the Combine Alcian Blue -PAS and Hematoxylin and Eosin in the Detection of Gastric Intestinal Metaplasia among Sudanese Patients with Chronic Gastritis
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Date
2022-06-14
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جامعة الشيخ عبدالله البدري
Abstract
ABSTRACT
Gastric cancer is the fifth most common cancer and the second most frequent cause of cancer death worldwide, gastric cancer remains a major cause of
mortality and morbidity worldwide. Gastric intestinal metaplasia, an intermediate step in gastric carcinogenesis, is generally regarded as a pre-malignant
lesion. Gastric intestinal metaplasia is a precancerous modification of the mucosa of the stomach with intestinal epithelium and is associated with an expanded
danger of dysplasia and cancer. The study aimed to compare and evaluate the combined alcian blue –PAS and Hematoxylin and Eosin, in the detection
of gastric intestinal metaplasia among Sudanese patients with chronic gastritis. Two hundred tissue sections were cut from 100 formalin-fixed paraffin embedded Blocks (n=100) were collected from patients who attended El-mek Nimir University Hospital & Specialized Centers at Shendi locality- River
Nile State between June to December 2021, and thereafter processed and examined histopathologically, all blocks were cut into (3 micrometers) by using
SLEE-MAINZ,-CUT4060 microtome. And spread in a water bath then put in coated glass slides, then all sections deparaffinized in xylene and rehydrated
through descending grade of alcohols, then water.100 tissue sections were stained by hematoxylin and eosin technique and 100 were stained by combined
alcian blue –PAS stain technique. Combine Alcian blue & PAS is more detected the Gastric Intestinal Metaplasia compared to H& E was observed among
chronic gastritis patients in Combine Alcian blue & PAS technique positive in 19 (19%) cases and negative in 81(81%) , by routine H& E is positive in 11
(11%) , cases and negative in 89 (89%) cases and the overall prevalence of gastric intestinal Metaplasia was 19%, from which 12 (63.1%) among female, and
7 (36.8%) were male and the age they classify into three groups A (10-40 years) group B (41-70 years) group C (71-100 years) the result of GIM positive
rate was found 2 (6.6) , 10(23.3%) , 7 (25.9%) respectively. On the other hand, the sensitivity of H& E in the detection of (GIM) was 70.3 % and specificity
was 100%. The study concluded that combining Alcian blue & PAS is more detected and sensitive in the detection of gastric intestinal metaplasia among
Sudanese Patients with chronic gastritis compared to H& E. Additionally, The most positive rate was among females and within the age group (41-70) years
old was observed. This may direct the clinicians and researchers to it could be better to use combined Alcian blue & PAS in diagnosing Gastric Intestinal
Metaplasia and look for more information about the detection of gastric intestinal metaplasia