Bacterial Keratitis in Contact Lens Wearers in Khartoum
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Date
2021-09-05
Journal Title
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Publisher
جامعة الشيخ عبدالله البدري
Abstract
Abstract: Background: In both industrialized and developing nations, contact lenses have become a popular substitute for
eyeglasses. However, unpleasant reactions can develop during contact lens usage in some cases, and a variety of
microorganisms—including bacteria, fungus, and free-living amoebae—may cause a variety of eye infections. The goal of this
study was to identify contaminated bacteria from contact Wearers. Method: With the use of sterilized cotton swabs, samples
were taken from eye lens solution bottles and cultivated directly on solid medium. All samples were inoculated onto blood agar
and MacConkey's agar and cultured at 37°C for 24-48 hours. Cultures were declared negative if no growth was detected after
48 hours. On the basis of culture diagnosis by growing on medium and completing biochemical tests, bacteria were identified
using Gram's staining. A structured interview questionnaire was used to collect data, which included demographic data as well
as risk variables. For data input and analysis of the patient demographic information, the Statistical Package for Social Sciences
application (SPSS Inc., Chicago, IL, USA) version 20 was used. Simple descriptive statistics were used to assess the results of
the culture, biochemical test, gram stain, and demographic data. Every two variables were compared using the Chi-square test.
A statistically significant p value was less than 0.05. Result: A total of 150 samples were taken from an aqueous solution of
contact lenses in order to isolate microorganisms. Bacteria were isolated from 84 samples in an aqueous solution, whereas 66
samples were found to be growth negative. Pseudomonas aeurogenosa was the most often isolated bacterium, with 39 (46 %),
Stapylococcus epidermidis 27 (32%), Staphylococcus aureus 12 (14 %), and Escherichia coli 6 (7%), respectively. Conclusion:
The research demonstrates a range of bacteria in the contact lens solution under evaluation, with pseudomonas bacteria being
the most frequent. In addition, users who shared contact lenses had more isolated bacteria encounters. The most preventive
factor was found to be high compliance with lens care practices, whereas inadequate compliance with hand washing before
wearing lenses was associated to a high contamination rate.