Browsing by Author "Abdelhakam H. Ali"

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    Isolation of the Pathogenic Bacteria from Banknotes and Coins in Khartoum City Pre-COVID-19 Era, Sudan
    (جامعة الشيخ عبدالله البدري, 2020-12-20) Abdelhakam H. Ali; Algawhara Tagalsir Abdalrahman; Ashwag Mohammed Ahmed; Duaa Haj Ali Hajhamed; Maali Bilal Ahmed Abdalrazig; Mawada Hassan Fadllalah Mohammed; Mosab Nouraldein Mohammed Hamad; Mogadam Bahar
    ackground: Coinage notes and moneys are widely exchanged between various communities for trade, which may serve as a carrier of microbes and lead to transmission of infectious diseases. The aim of this study is to isolate microbial contamination of Sudan currency notes and coins that life threatening condition of Sudanese people. Methods: One hundred samples (48 currency notes and 52 coins) were collected from various place of society namely, bus conductor, cafeteria, vegetable seller and pharmacies to reveal the bacteria profile of circulating coins and currency notes. Results: Out of the total number of all isolates from all the coins and currency put together was 169, of which the pathogenic isolate count 62(36%) organism Staphylococcus aureus 28(17%), Klebsiella pneumonia 14(8%), Enterobacter species 9(5%), Enterococcus faecalis 6(4%), Pseudomonas aeruginosa 3(2%), Escherichia coli 2(1%). While the remnant which consider as Normal flora was form 107 (64%) organism Bacillus species 33 (20%), Staphylococcus epidermidis 24(14%), Micrococcus Species 1(1%) and fungi was present 49(29%) of all isolates. The result of sensitivity test toward the pathogenic isolates showed that overall isolates Exhibited high resistance to vancomycin and Penicillin, while absent or little resistance was against antibiotics like, gentamicin, ciprofloxacin, and tetracycline. Conclusion: The study suggested that Sudanese paper currency and coins are highly contaminated with pathogenic microorganisms and this contamination may play a significant role in transmission of infectious diseases. Hence, great care must be taken while handling money during the preparation and handling of food to avoid cross contamination.
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    olecular Detection of Colistin Resistance Gene mcr-1 in Gram negative Rods Isolated from Hospitalized Patients in Khartoum State
    (جامعة الشيخ عبدالله البدري, 2020-08-17) Areej Zakaria; Zeinab Mohammed Abdo; MF Ismail; Abdelhakam H. Ali; Mosab Nouraldein Mohammed Hamad; Mawada Rhmtallah
    Abstract Background: The emergence of the plasmid-mediated mcr-1 colistin-resistance genes in bacteria poses a potential threat for treatment of patients, especially when hospitalized. The pinpoints of this study were to search for the presence of mcr 1genein the thick of gram negative rods (Eschericea coli, Klebsiella spieces, Pseudomonas speices, Proteus speices, Aceinetobacter species, Serritia and Citrobacter species) isolates from clinical specimen using Polymerase Chain Reaction (PCR) technique. Methods: In this study, 185 nonduplicate Enterobacteriaceae isolates from clinical specimens were tested. Antimicrobial susceptibility test on 11 antibiotics was done by Kirby baur disk diffusion method. The presence of mcr-1 gene was investigated by PCR technique. Results: Out of 185 non duplicate Enterobacteriaceae, 6 isolates show positive mcr-1 gene including one Isolate (16.4%) Pseudomonas aeurginosa from urine sample, and 5 (83.3%) Klebsiella pneumonia isolated from two wound sample (40%), one high vaginal swab sample (20%), one blood sample (20%) and one urine sample (20%). Most of Isolates that harboring mcr-1 gene detected by PCR isolated from patients were used antibiotics for treatment (n=4 (66.6%)). Conclusion: Colistin (Polymycin E) which used for treating Gram- negative bacterial infections should be mainly used for treating the severest infection to preserve their effectiveness and to avoid transferring of the gene from one bacterium to other by the different methods of genes transferring.
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    Seroprevalence of Rotavirus among Selected Group of Sudanese Children
    (جامعة الشيخ عبدالله البدري, 2022-04-02) Abdelhakam H. Ali; Huzafia Abdallah Khalid Saad; Mossab Balla Abdallah Abdalrahem; Mosab Nouraldein Mohammed Hamad
    Abstract: Rota virus is the second causes of diarrhea among childrenIn Sudan [2]. The current study was conduct to detect rotavirus gastroenteritis among vaccinated children Methodolgy: A total of59 diarrheal samples was collected from children with acute diarrhea admittedto Mohammed AL Amin Hamid paediatric Hospital throw period from October to November 2019, ELISA was dine to detect Rota virus antigen. Result: Out of 59 samples 84.7% was vaccinated were 15.3% was unvaccinated.in total of 50 vaccinated children 30% is re-infected with rota virus with acute to chronic illness and show positive antigen with ELISA. Conclusions: vaccinated children is infected with rota virus and symptoms is occurs samples for virus antigen was record positive result in ELISA this may related to other genotype or serotype or mutation.