Samar Osman AliAbel Basit Osman HroonMosab Nouraldein Mohammed Hamad*2023-11-272023-11-272019https://ds.eaeu.edu.sd/handle/10.58971/554ABSTRACT Visceral leishmaniasis is an endemic disease in Sudan which affects many populations certainly in Gadarif State. A hospital-based cross-sectional study was conducted at Gadarif Teaching Hospital, 100 healthy apparent blood donors were included in the study and their blood specimens were examined microscopically by buffy coat technique and serologically by rk39 and there was no positivity reported in the study. Further study must be conducted with large sample size and molecular diagnostic method (PCR) must be used.other