Waseem Sameer KwamiShamsoun Khamis KafiMahdi Hassan MahmoudAbdul Hakam Hassan AldigealMosab Nouraldein Mohammed Hamad2023-11-272023-11-272020-06-26https://ds.eaeu.edu.sd/handle/10.58971/540Abstract Diarrhea remains the first leading causes of children mortality worldwide specially in developing countries, lead to approximately (1.87) million deaths yearly.There is a global agreement that rotaviruses are the single prominent causative agents of childhood diarrhea illnesses worldwide. The objective of this work was to characterize human rotavirus strains circulating among children attended with diarrhea to Mohammed Alamin Hamid Pediatric Hospital using molecular methods. 150 fecal specimens from infants less than 5 years were collected and analyzed. The samples were investigated for presence of rotavirus using antigen based enzyme immune-sorbent assay (ELISA), genotyping was carried out by RT-PCR to determine virus genotypes using VP7 gene specific primer sets. Rotavirus was detected in stool of 42(28.7%) out of 150 total children. The study revealed that the percentage of (Rotavirus Ag positive) children was (29.3%) in males which is nearly equal to the percentage of Rotavirus Ag positive children in females (26.6%) but this difference in gender was significant statistically insignificant.other