Measurement of Platelet Parameters and D-Dimer Level in Sudanese Patients with Long Standing Diabetes Mellitus Type 2
No Thumbnail Available
Date
2020-02-06
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
جامعة الشيخ عبدالله البدري
Abstract
Background: This is an observational analytical case control study that had been conducted among diabetic patients in Khartoum
State, during the period from December to February 2016 - 2017, to compare platelets parameters and D-dimer level between dia betic patients attending Diabetic and Endocrine Hospital, Soba University Hospital and non-diabetic controls. 80 blood samples were
collected 40 samples from diabetic patients and 40 samples from non-diabetic controls.
Objectives: To evaluate platelet parameters and D-Dimer level in long standing Diabetes mellitus type 2 in Sudanese patients.
Method: After obtaining informed consent from each patient and non-diabetic control, 4 ml of venous blood were collected and
divided into two parts: 2.5 ml transferred into K2EDTA container for complete blood count, and 2.5 ml transferred into Tri Sodium
Citrate container (9 volumes of blood is added to 1 volume of a 32 g/1 solution of TSC anticoagulant) and separated by centrifugation
at 3000 rpm for 15 minutes, to obtain platelet poor plasma for measurement of D-Dimer level. The following tests were performed
(platelets count, MPV, PDW, P-LCR and D-Dimer level) in both groups (case and control).
Result: Platelets count in both case and control was statistically insignificant (p = 0.392), while MPV (P = 0.034), PDW (p = 0.020), P CLR (p = 0.012), and D-Dimer. Level (p = 0.015) were statistically significantly increased in patients compared to controls. There were
no statistically significant differences in all measured parameters between gender, age and duration groups except platelets count
which was significantly increased in patients under 50 years old. P-value < 0.05 was considered significant throughout the study.
Conclusion: Our study identified the altered platelets morphology and increased D-Dimer level among the long standing DM type 2
which are indicators of thrombotic state in diabetes mellitus. Based on these results we recommend the platelets indices and D-dimer
level to the spectrum of thrombophilia which will be carried on long standing diabetes mellitus type 2 patients.