COLORIMETRIC DETECTION OF PLASMODIUM VIVAX IN PATIENT’S URINE USING MSP10 OLIGONUCLEOTIDES AND GOLD NANOPARTICLES

Abstract

ABSTRACT Background: In Sudan and elsewhere, diagnosis of malaria relies entirely on examination of blood taken from suspected individuals. In this study an attempt to examine urine for detection of P. vivax infection is carried out. Methodology: A total of 35 urine samples from P. vivax positive patients and 25 negative individuals were heated by thermocycler and cooled were mixed with P. vivax merozoites surface protein 10(MSP10) and AuNPs. Results: The results showed that the urine of all P. vivax positive patients turned red color due to stabilization of the gold nanoparticles. Conclusion: this indicate that MSP10 and nanoparticles are sensitive enough to detect P. vivax and can consequently be recommended as diagnosis techniques.