Detection of Bacterial Contaminants from Operating Theatres at Hospitals in Shendi City, Sudan

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جامعة الشيخ عبدالله البدري
Abstract: Background: Operating room contamination is recognized as one of the most common life-threatening microbial contaminations in hospital environments, especially operating rooms and other specialty units, and is an ever- increasing cause of nosocomial infections. Objective: The purpose of this study was to isolate and identify bacterial contaminants in Shendi hospital operating room. Between July-September 2021. Materials and Methods: Sixty samples were collected from various locations in the operating room and all isolated bacteria were identified. The study isolated five types of bacteria from the Shendi Hospital operating room. Results: The results showed that Micrococcus was the most common bacterial contaminant isolated from the operating room. Leteus 21 (32.3%) Staphylococci. Epidermis 16 (24.6%), Staphylococci aureus 11 (16.9%), Bacillus sp. 9 (13.8%), Bacillus cereus 8 (12.3%)) and the lowest contaminants were isolated from soil 15 (23%), and focus lamp 5 (7.7%). This study may point to the fact that the Shendi Hospital operating room had bacterial contamination that could lead to postoperative wound infections (SSI). Reasons for contamination may be due to excessive attendance, personnel movement, and ineffective sterilization and disinfection procedures. Conclusions: Bacterial contamination was highest in operating room beds, followed by carts, floors, and focused lambs. Micrococcus spp, S. epidermidis, S. aureus, and Bacillus species have proven to be the most serious contaminants in the operating room, and are a dangerous cause of hospital-acquired infections, killing patients and hospital staff threatening. This may indicate that the sterilization method is not efficient enough, putting the patient at risk for postoperative infection. Some organisms were resistant to Gentamicin and highly sensitive to Imipenem, Ciprofloxacin, and Ceftriaxone