In vitro Assessment of Antimicrobial Activity of Honey Bees and Nigella sativa Against Selected Clinical Isolates from Shendi City

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جامعة الشيخ عبدالله البدري
Abstract Background: An alarming rise in bacterial strains resistant to existing antimicrobial agents requires a renewed effort to seek agents effective against pathogenic bacteria resistant to antimicrobials. The new antimicrobial agents are will overcome this problem. Objective: This study aimed to study the in vitro antimicrobial activity of different concentrations of Honey, petroleum ether extracts of medicinal plant Nigella sativa (seeds), and the mixture using the cup-plate agar diffusion method. Materials and Methods: This cross-sectional observational study was carried out among One hundred samples were collected from urine, wound, ear, and eye swab, 95/100 (95%) showed bacterial growth, from which six types of pathogenic bacteria at Shendi city hospitals during March to June 2018. Results: Of the total 95 clinical and standard specimens were confirmed as Staphylococcus aureus 20 (21%), Escherichia coli 20 (21%), Pseudomonas aeruginosa 15 (16%), and Klebsiella pneumoneae 30 (31%). In addition to Candida albicans 10 (11%). These results showed the activity of antimicrobial Petroleum ether extract of the Nigella sativa, and honey pronounced dose-dependent on standard strains and clinical isolates, while methanolic extract of Nigella sativa explained no activity. Conclusion: The activity of Nigella sativa, honey, and the mixture exhibited high antimicrobial activity against all types of tested or ganisms both clinical and standard organisms. Therefore Nigella sativa, honey, and the mixture can be regarded as a broad-spectrum antimicrobial agent.