IDENTIFICATION OF CHEMICAL CONSTITUENTS, ANTIMICROBIAL AND ANTIOXIDANT ACTIVITY OF CITRULLUS COLOCYNTHIS SEEDS OIL
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Date
2020
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جامعة الشيخ عبدالله البدري
Abstract
ABSTRACT
This study aimed to extract seeds oil of Cirtullus colocynthis and to identify the chemical constituents of the medically
important species Citrullus colocynthis seeds oil and to evaluate its potential antimicrobial activity. The seeds were
collected from El-Obeid locality, North Kordofan State December (2018). The extraction of seeds oil was carried out at
Standards and Metrology Laboratories in (El-Obeid) then analyzed at National Research Center in (Khartoum). 17
components of Citrullus colocynthis seeds oil extract were detected by Gas Chromatography-Mass spectroscopy (GC MS) method. The results showed that the major components are: 9, 12-octadecanoic acid methyl ester (54.52 %);
hexadecanoic acid methyl ester (15.39%); methyl stearate (14.24%) and 9-octadecanoicacid methyl ester (12.53%). The
antimicrobial activity of the extracted seeds oil was evaluated using diffusion assay against Gram positive bacteria:
Staphylococcus aurous, Bacillus subtilis and Gram negative bacteria: Escherichia coli, Pseudomonas aeruginosa, and
Fungus Candida albicans. The oil extract showed that highly activity against Fungus Candida albicansat concentration
100mg/ml and poor activity at concentration 75mg/ml but no distinct inhibition zone was revealed at both concentration
50mg/ml and 25mg/ml. For four types of bacteria at concentration 100mg/ml extracted seeds oil was partially active
against four types of bacteria. More over at concentration 75mg/ml partially activity was exhibited against Bacillus
subtitles Pseudomonas aeruginosa, but poor activity was shown against Escherichia coli and Staphylococcus aureus. So
far Bacillus subtitles showed partially activity at concentration 50mg/ml and 25mg/ml but poor activity was exhibited
against Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli. The antioxidant activity of the extracted
oil was evaluated by using the standard 2, 2diphenyl-1-picrylhydrazyl (DPPH) 0.5 ml. The antioxidant activity of the
extracted oil was 39 ± 0.8. This study Recommend to test Antimicrobial activity of this plant against other types of
bacteria and fungus and Analysis of other parts of Citrullus colocynths