Browsing by Author "Waseem Sameer Kwami"

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    Molecular Characterization of Human Rotavirus Strains Circulating Among Children Less Than 5 Years Attended With Diarrhea to Mohammed Alamin Hamid Pediatric Hospital, Khartoum
    (جامعة الشيخ عبدالله البدري, 2020-06-26) Waseem Sameer Kwami; Shamsoun Khamis Kafi; Mahdi Hassan Mahmoud; Abdul Hakam Hassan Aldigeal; Mosab Nouraldein Mohammed Hamad
    Abstract Diarrhea remains the first leading causes of children mortality worldwide specially in developing countries, lead to approximately (1.87) million deaths yearly.There is a global agreement that rotaviruses are the single prominent causative agents of childhood diarrhea illnesses worldwide. The objective of this work was to characterize human rotavirus strains circulating among children attended with diarrhea to Mohammed Alamin Hamid Pediatric Hospital using molecular methods. 150 fecal specimens from infants less than 5 years were collected and analyzed. The samples were investigated for presence of rotavirus using antigen based enzyme immune-sorbent assay (ELISA), genotyping was carried out by RT-PCR to determine virus genotypes using VP7 gene specific primer sets. Rotavirus was detected in stool of 42(28.7%) out of 150 total children. The study revealed that the percentage of (Rotavirus Ag positive) children was (29.3%) in males which is nearly equal to the percentage of Rotavirus Ag positive children in females (26.6%) but this difference in gender was significant statistically insignificant.
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    Prevalence of human immunodeficiency virus among tuberculosis patients in Khartoum state
    (جامعة الشيخ عبدالله البدري, 2020-06-30) Waseem Sameer Kwami; Abass Ahmed Ali Ahmed; Mosab Nouraldein Mohammed Hamad
    Abstract Introduction: Pulmonary Tuberculosis (TB) and human immunodeficiency virus (HIV) have been closely connected since the appearance of acquired immunodeficiency syndrome (AIDS). HIV infection among active TB cases provides real challenges in diagnosis and treatment of TB. This is descriptive cross-sectional study conducted at Abu-anja Teaching Hospital; Khartoum state in period from October 2018to March 2019.The objective was to determine the prevalence of Human Immunodeficiency Virus infection among tuberculosis patients. Method: A total of 90 venous blood samples were collected from the study participants, serum was obtained by centrifugation at (3000 rpm) for 5 minutes. The levels of HIV antibody titer were determined by using sandwich ELISA Assay. Results: The studies revealed that out of the 90 participants of the tuberculosis patients, 5(5.6%) were HIV positive, and the HIV prevalence among male patients were significantly higher than female patients. Conclusion: The study concluded that there is high prevalence of HIV among tuberculosis patients in Khartoum state, capital of Sudan (5.6%) comparing with (5%) at which the WHO recommended intensified intervention to address TB-HIV co-infection critical in any strategy that aims to reach those most in need.
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    Screening the Antibacterial Activity of Moringa Oleifera Leaves and Seeds Extract against Selected Members of Bacteria
    (جامعة الشيخ عبدالله البدري, 2020-08-17) Waseem Sameer Kwami; , Hamodi Ahmed Saeed; Mosab Nouraldein Mohammed Hamad
    background: Natural antibacterial agents derived from different parts of medicinal plants (leaves, seeds, roots, flowers….etc) might represent an alternative treatment of infectious diseases, Plants with their wide variety of chemical constituents offer a promising source of new antimicrobial agents, with general as well as specific antibacterial activity. The aim of this study is to screen antibacterial activity of Moringa oleifera leaves and seeds extract against selected members of bacteria. Method: Five bacterial ATCC strains and seven clinically isolated strains were used in this study. 100 g of Moringa olifara leaves and 150 g of the coarsely powdered Seeds were extracted with 80 % methanol using soxhlet extractor apparatus for about eight hours. The antibacterial activity was determined by the agar well diffusion method .Muller Hinton agar medium was used for bacterial cultures. Results: The study reveals that the Moringa oleiferaleaves and seeds extract show prominent Invitro antibacterial activity against all used bacterial species and the antibacterial activity of leaves extract is stronger than antibacterial activity of seeds extract and these results showing the possibility of using this tree in treatment of diseases caused by these pathogens. Conclusion: The study concluded that the activity of this plant in the treatment of diseases associated with bacterial pathogens is validated and scientifically supported by the results obtained in this study.