Browsing by Author "Mosab Nouraldein Mohammed Hamad"

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    Detection of Extended Spectrum Beta-Lactamase (ESBLs) among Urinary Tract Patients in Khartoum State, Sudan
    (جامعة الشيخ عبدالله البدري, 2023) Leila M. Ahmed Abdelgader; Mosab Nouraldein Mohammed Hamad; Tibyan Abd Almajed Altaher; Ghanem Mohammed Mahjaf; Areej Osman Shik Aldeen
    ABSTRACT Background: Many different organisms can cause urinary tract infections, but Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Enterococcus faecalis, and Staphylococcus saprophyticus are the most prevalent ones. 60% of all antibiotics used to treat infectious diseases around the world are beta-lactam antibiotics, one of the main classes used to fight gram-negative and gram-positive bacteria. Objective: The purpose of this study is to detect and isolate Extended-Spectrum Beta-Lactamases (ESBLs), which are responsible for urinary tract infections, as well as check for any potential medication resistance. Materials and Methods: The gram stain technique and biochemical assays were used to identify 100 urine samples from Khartoum state hospitals based on their cultural characteristics and morphological appearance. Using the disk diffusion method, the isolates were tested for antimicrobial susceptibility to third-generation cephalosporins (Cefotaxime, Ceftazidime, and Ceftriaxone). The Combination Disk Technique (clavulanic acid+third-generation cephalosporins) was used to inoculate the bacterial isolates to demonstrate their capacity to create ESBL. In comparison to non-ESBL producers, the ESBL producers were assessed. Results: E. coli, Klebsiella, S. aureus, Proteus, and Pseudomonas were among the microorganisms isolated from UTI patients. 61% of the participants in this study were female, whereas 39% were male. E. coli has an increased frequency among isolated bacteria, as it presented in 46% of urine cultures, followed by pseudomonas and Klebsiella, each with a 22% frequency. Growth of the majority of the bacteria was found among females more frequently than males, and it also seems to be among older age patients than younger. Amoxyl alone and in combination with Clavulanic Acid (AAMC) was the most medicine that bacteria were resistant to (76%), but Ceftriaxone (CTR) has higher sensitivity (45%) and resistance (50%). The growth of the bacteria in the media of antibiotics was sorted into sensitive, intermediate, and resistant. Conclusions: Tested antibiotic resistance was higher for AAMC than for CTR, which is typically taken without performing urine sample culture and sensitivity testing, which over time leads to increased resistance.
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    Molecular Characterization of Human Rotavirus Strains Circulating Among Children Less Than 5 Years Attended With Diarrhea to Mohammed Alamin Hamid Pediatric Hospital, Khartoum
    (جامعة الشيخ عبدالله البدري, 2020-06-26) Waseem Sameer Kwami; Shamsoun Khamis Kafi; Mahdi Hassan Mahmoud; Abdul Hakam Hassan Aldigeal; Mosab Nouraldein Mohammed Hamad
    Abstract Diarrhea remains the first leading causes of children mortality worldwide specially in developing countries, lead to approximately (1.87) million deaths yearly.There is a global agreement that rotaviruses are the single prominent causative agents of childhood diarrhea illnesses worldwide. The objective of this work was to characterize human rotavirus strains circulating among children attended with diarrhea to Mohammed Alamin Hamid Pediatric Hospital using molecular methods. 150 fecal specimens from infants less than 5 years were collected and analyzed. The samples were investigated for presence of rotavirus using antigen based enzyme immune-sorbent assay (ELISA), genotyping was carried out by RT-PCR to determine virus genotypes using VP7 gene specific primer sets. Rotavirus was detected in stool of 42(28.7%) out of 150 total children. The study revealed that the percentage of (Rotavirus Ag positive) children was (29.3%) in males which is nearly equal to the percentage of Rotavirus Ag positive children in females (26.6%) but this difference in gender was significant statistically insignificant.
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    Prevalence of Bacterial Contamination on Mobile Phones of Medical Staff in Shendi Hospitals-Sudan
    (جامعة الشيخ عبدالله البدري, 2021-09-10) Neimat Osman Bashir; Marwa Mohamed Ali Gaid; Hiba Alla Mohammed Ahmed; Esraa Fateh Alrhman Abdaelqader; ManahiBalla Jomaa; SuhaAbdalmannan Badwi; Haghamad Allzain; Omran M. Ahmed; MazinBabekir Musa; Mohamed AhmedIbrahim Ahmed; Nahla Ahmed Mohammed Abdelrahman; Mosab Nouraldein Mohammed Hamad; Nassreldeen Khalid Abdelrahman
    Abstract: Back ground: The goal of this cross-sectional descriptive study was to isolate and identify several bacterial species in mobile phones among hospital staff at Almak Nimer University Hospital and Shendi Teaching Hospital in Shendi town, River Nile State, North of Sudan. Between December 2019 and November 2020, the research was carried out. Methodology: One hundred (100) swabs were randomly taken from the phones of male and female medical staff volunteers from Almak Nimer University Hospital and Shendi Teaching Hospital, Blood agar and MacConkey agar were used to grow the specimens. Finally, colonial morphology, gram stain, and biochemical tests were used to identify the isolated bacteria. Results: Eighty-seven (87) mobile phones were discovered to be cultured positive in the research (87 %). The isolated organisms were: S. aureus (28; 32%), Coagulase gram negative staphylococci (26; 30%), Bacillus spp (16; 18%), P. aeruginosa (7; 8%), K. pneumonia (5; 6%), E. coli (4; 4%) and Diphteroid (1; 1%). Laboratory technicians (93%) were the most likely to be contaminated, followed by nurses (88%), physicians (80%), and pharmacists (50%). In terms of gender contamination, females account for 87%, while males account for (86 %). Conclusion: The results of this study showed that medical staff's phones might be a source of nosocomial infection.
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    Seroprevalence of toxoplasmosis between aborted ladies in Atbara district, Sudan
    (جامعة الشيخ عبدالله البدري, 2019-02-12) Rayan Khalid Mohamed Taha; Mosab Nouraldein Mohammed Hamad; Khalid Mohamed Taha; Ali Elamin Nasir Hamad
    Abstract Background: Toxoplasma gondii is an oblige intracellular protozoan parasite leading to toxoplasmosis in animals and humans. Principal maternal infection with toxoplasmosis throughout pregnancy is frequently associated with vertical transmission to the fetus. However it is not convinced whether Toxoplasma infection can cause frequent abortion. Justification: There is no formerly published data about the occurrence of toxoplasmosis between aborted women in Atbara district. Objectives: To recognize the Seroprevalence of toxoplasmosis among aborted women in Atbara district Method: Cross sectional, descriptive study, 152 aborted women from Atbara district were examined for anti-Toxoplasma gondii antibodies, three diagnostic techniques were used (latex agglutination, ICT and ELISA). Result: Generally the Seroprevalence rate of Toxoplasma parasite between aborted women according to the techniques used; 33.6 % by latex agglutination, 22.4 % by ICT and 35.5 % by ELISA method. Conclusion: The study conclude that there was high prevalence of toxoplasmosis among the participants and indicated that ELISA is the best serological method for detection of Toxoplasma gondii infection.