Browsing by Author "El-badri E. Osman"

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    Antiamoebic activity and cytotoxicity of ethanolic extract of Cyperus rotundus L.
    (جامعة الشيخ عبدالله البدري, 2015) El-badri E. Osman
    ABSTRACT Cyperus rotundus L. (Family: Cyperaceae), has a number of pharmacological and biological activities including anti-inflammatory, antidiabetic, antidiarrhoeal, cytoprotective, antimutagenic, antimicrobial, antioxidant, cytotoxic and apoptotic, anti-pyretic and analgesic activities. The World Health Organization (WHO) estimates that the protozoan Entamoeba histolytica is a major cause of morbidity worldwide, causing approximately 50 million cases of dysentery and 100,000 deaths annually. Intestinal amoebiasis due to the infection of E. histolytica is ranked third on the list of parasitic protozoan infections leading to death behind malaria and schistosomiasis. The purpose of the paper was to investigate the in-vitro antiamoebic activity and cytotoxicity (MTT assay) of ethanol extract of C. rotundus L. (whole plant). The ethanol extract of C. rotundus (whole plant), with different concentration (500, 250 and 125 ppm) and metronidazole concentration (312.5 μg/ml) was investigated in vitro against E. histolyica trophozoites. The result was obtained from C. rotundus whole plant ethanol extract which exhibited 100% mortality within 96 h, at a concentration 500 ppm; this was compared with Metronidazole which gave 96% inhibition at concentration 312.5 µg/ml at the same time. In addition cytotoxicity (MTT assay) with different concentration (500, 250 and 125 ppm) in comparison to triton-x100 (the reference control) which verified the safety of the examined extract with an IC50 less 100 µg/ml. In conclusion, these studies prove the potent activity of C. rotundus against E. histolyica trophozoites in vitro with verified safety evidence for use.
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    Antigiaridial Activity and Cytotoxicity of Ethanolic Bark Extract of Acacia nilotica (L.)
    (جامعة الشيخ عبدالله البدري, 2016) El-badri E. Osman
    Abstract: Background: Acacia nilotica (L.) was used to treat different ailments for instance cold, congestion, fever, gallbladder, hemorrhage, hemorrhoids, leucorrhoea, ophthalmic, sclerosis, small pox, intestinal pains and acute diarrhea. Other preparations are used for gargle, toothache, ophthalmic and syphilitic ulcers. Human parasitic infections still represent a challenging public health problem worldwide, especially in tropical and subtropical regions. Giardiasis is the most common cause of parasitic gastro-intestinal disease and up to two hundred million people are chronically infected with Giardia lamblia globally with 500,000 new cases reported annually. Objectives: The purpose of the paper is to investigate the in-vitro antigiardial activity and cytotoxicity (MTT assay) of ethanol extract of A. nilotica subsp. nilotica (bark). Method: The ethanolic extract of A. nilotica (bark), with different concentrations (500, 250 and 125 ppm) were investigated together with Metronidazole as a reference control at 312.5 μg/ml against Giardia lamblia trophozoites. Cytotoxicity (MTT assay) was also performed with different concentrations (500, 250 and 125 ppm) and compared to the reference control Triton-x100. Result: A. nilotica bark ethanolic extract exhibited 100% mortality within 96 h, at a concentration of 500 ppm; this was compared with Metronidazole which gave 96% inhibition at the concentration of 312.5 μg/ml at the same time. In addition cytotoxicity (MTT-assay) verified the safety of the examined extract with an IC50 less than 100 μg/ml. Conclusion: These studies prove the potent activity of A. nilotica against Giardia lamblia trophozoites in vitro with verified safety evidence for use. .
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    In Vitro Antiprotozoal Activities and Cytotoxicity of Selected Sudanese Medicinal Plants
    (جامعة الشيخ عبدالله البدري, 2017) El-badri E. Osman
    Abstract: Entamoeba histolytica is ranked third on the list of parasitic protozoan infections leading to death behind malaria and schistosomiasis. It is estimated also up to 200 millions of people are chronically infected with Giardia lamblia globally. Metronidazole is the drug currently widely used and recommended in the treatment of amoebiasis and giardiasis. However; sometimes it causes adverse effects such as myoplasia, neuralgia, allergic dermatitis, and others. The in vitro antiprotozoal activities of some selected Sudanese medicinal plants (Acacia nilotica subsp. nilotica, Adansonia digitata, Cyperus rotundus and Nigella sativa) were determined against Entamoeba histolytica and Giardia lamblia by employing the sub-culture method. The mammalian cytotoxicity of the investigated plants against normal Vero cell line was determined by applying MTT [(4, 5- dimethyl thiazol-2-yl)-2, 5-diphenyl tetrazolium bromide] method. All plants examined 100% inhibition at a concentration 500 µg/ml after 96 h; this was compared with Metronidazole which gave 95% inhibition at concentration 312.5 µg/ml at the same time against Entamoeba histolytica and Giardia lamblia. In addition, cytotoxicity (MTT-assay) of these plants against normal Vero cell line which verified the safety of the examined extracts with an IC50 less 100 µg/ml. These studies prove the potent activity of extracts against E. histolyica and G. lamblia trophozoites in vitro with verified safety evidence for use.